Biography

Bojana Gligorijevic, PhD, graduated Chemistry at University of Belgrade with an honors thesis in analytical chemistry, isolating active compounds from endemic plants. At Georgetown University, Dr. Gligorijevic’s PhD chemistry thesis took a turn when she first saw a fluorescent microscope. She ended up developing new technologies for automated 4D imaging and analysis of malaria division and metabolic processes. During her postdoctoral training in cancer biology at Albert Einstein College of Medicine, she developed new tools for intravital imaging in mouse models and contributed to the current view of tumor cell intravasation mechanism in tumor microenvironment context.

Research Interests

  • Intravital imaging
  • Multiphoton Microscopy
  • Machine Learning Image Classification
  • Fluorescent Proteins
  • Cancer Complexity
  • Cell Decision-making
  • Extracellular Matrix Remodeling
  • Tumor Microenvironments
  • Mouse Models of Metastasis
  • Invadopodia
  • Motility & Invasion.

Courses Taught

Number

Name

Level

BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology

Undergraduate

BIOE 4555

Capstone Elective - Biophotonics: Seeing is Believing

Undergraduate

BIOE 5555

Biophotonics: Seeing is Believing

Graduate

BIOE 5600

Bioengineering Graduate Seminar

Graduate

Selected Publications

Recent

  • Perrin, L., Tucker, T., & Gligorijevic, B. (2021). Time-resolved fluorescence imaging and analysis of cancer cell invasion in the 3d spheroid model. Journal of Visualized Experiments, 2021(167). doi: 10.3791/61902

  • Firestein, R., Marcinkiewicz, C., Nie, L., Chua, H.K., Quesada, I.V., Torelli, M., Sternberg, M., Gligorijevic, B., Shenderova, O., Schirhagl, R., & Feuerstein, G.Z. (2021). Pharmacodynamic studies of fluorescent diamond carriers of doxorubicin in liver cancer cells and colorectal cancer organoids. Nanotechnology, Science and Applications, 14, pp. 139-159. doi: 10.2147/NSA.S321725

  • Gligorijevic, B. (2020). Real-time, high-resolution microscopy of invasive cancer cells in the tumor microenvironment context. Journal of Biomolecular Techniques : JBT, 31, p. S40.

  • Perrin, L., Bayarmagnai, B., & Gligorijevic, B. (2020). Frontiers in intravital multiphoton microscopy of cancer. Cancer Reports, 3(1). doi: 10.1002/cnr2.1192

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2019). Invadopodia Dynamics is Regulated by ECM Cross-Linking. BIOPHYSICAL JOURNAL, 116(3), pp. 414A-414A. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E. & Gligorijevic, B. (2019). Machine Learning Classification of Cancer Cells Migration in 3D Multi-cue Microenvironments 1. 2018 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2018 - Proceedings. doi: 10.1109/SPMB.2018.8615608

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., Graña, X., Tüzel, E., & Gligorijevic, B. (2019). Invadopodia-mediated ECM degradation is enhanced in the G1 phase of the cell cycle. Journal of Cell Science, 132(20). doi: 10.1242/jcs.227116

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., & Gligorijevic, B. (2018). lnvadopodia are enriched in the G1 phase of the cell cycle. MOLECULAR BIOLOGY of the CELL, 29(26), pp. 227-227. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E., Bayarmagnai, B., Perrin, L., & Gligorijevic, B. (2018). Integrin beta 1 is the master regulator of the Invadopodia state in breast carcinoma cells. MOLECULAR BIOLOGY of the CELL, 29(26). Retrieved from http://gateway.webofknowledge.com/

  • Perrin, L. & Gligorijevic, B. (2018). EMT is decoupled from invadopodia and metastasis. MOLECULAR BIOLOGY of the CELL, 29(26), pp. 225-226. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E., Hoz, E.C.D.e.L.a., Cohen, A.R., & Gligorijevic, B. (2018). Contact guidance is cell cycle-dependent. MOLECULAR BIOLOGY of the CELL, 29(26). Retrieved from http://gateway.webofknowledge.com/

  • Soboloff, J., Gligorijevic, B., & Zaidi, M.R. (2018). STIM1 (c)AMPs up melanogenesis. The EMBO Journal, 37(5). doi: 10.15252/embj.201899047

  • Bergman, A. & Gligorijevic, B. (2018). Real-time imaging of invadopodia in tumor microenvironment context. Optics InfoBase Conference Papers, Part F89-MICROSCOPY 2018. doi: 10.1364/MICROSCOPY.2018.MF3A.3

  • Pourfarhangi, K.E., Bayarmagnai, B., Hoz, E.C.d.e.l.a., Cohen, A., & Gligorijevic, B. (2018). A methodology to investigate the relationship between cancer cells cell-cycle phase and their migratory behaviors. Optics InfoBase Conference Papers, Part F91-TRANSLATIONAL 2018. doi: 10.1364/TRANSLATIONAL.2018.JTu3A.13

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2017). Integrating live-cell fluorescent microscopy and signal processing to discover the relationship of invadopodia digging cycles with extracellular matrix crosslinking ratio. 2016 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2016 - Proceedings. doi: 10.1109/SPMB.2016.7846865

  • Perrin, L. & Gligorijevic, B. (2017). Role of Laminin Matrikines in Phenotypic Switching of Motile Cancer Cells. MOLECULAR BIOLOGY of the CELL, 28. Retrieved from http://gateway.webofknowledge.com/

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., & Gligorijevic, B. (2017). Invadopodia are limited to the G1 phase of cell cycle. MOLECULAR BIOLOGY of the CELL, 28. Retrieved from http://gateway.webofknowledge.com/

  • Bergman, A., Condeelis, J., & Gligorijevic, B. (2016). Invadopodium formation is enriched in perivascular niches and leads to cancer cell intravasation. CANCER RESEARCH, 76. doi: 10.1158/1538-7445.FBCR15-B05

  • Gligorijevic, B., Condeelis, J.S., & Bergman, A. (2014). Tumor cell motility in microenvironment context: From intravital microscopy to systems view. MOLECULAR BIOLOGY of the CELL, 25. Retrieved from http://gateway.webofknowledge.com/

  • Génot, E. & Gligorijevic, B. (2014). Invadosomes in their natural habitat. European Journal of Cell Biology, 93(10-12), pp. 367-379. doi: 10.1016/j.ejcb.2014.10.002

  • Bergman, A., Condeelis, J.S., & Gligorijevic, B. (2014). Invadopodia in context. Cell Adhesion and Migration, 8(3), pp. 273-279. doi: 10.4161/cam.28349

  • Gligorijevic, B., Bergman, A., & Condeelis, J. (2014). Multiparametric Classification Links Tumor Microenvironments with Tumor Cell Phenotype. PLoS Biology, 12(11). doi: 10.1371/journal.pbio.1001995

  • Hulit, J., Kedrin, D., Gligorijevic, B., Entenberg, D., Wyckoff, J., Condeelis, J., & Segall, J.E. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. Methods in Molecular Biology, 872, pp. 15-30. doi: 10.1007/978-1-61779-797-2_2

  • Gligorijevic, B., Bergman, A., Gil-Henn, H., & Condeelis, J. (2012). Microenvironments of tumor cell intravasation. CANCER RESEARCH, 72. doi: 10.1158/1538-7445.AM2012-2474

  • Gligorijevic, B., Wyckoff, J., Yamaguchi, H., Wang, Y., Roussos, E.T., & Condeelis, J. (2012). N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors. Journal of Cell Science, 125(3), pp. 724-734. doi: 10.1242/jcs.092726

  • Entenberg, D., Wyckoff, J., Gligorijevic, B., Roussos, E.T., Verkhusha, V.V., Pollard, J.W., & Condeelis, J. (2011). Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging. Nature Protocols, 6(10), pp. 1500-1520. doi: 10.1038/nprot.2011.376

  • Wyckoff, J., Gligorijevic, B., Entenberg, D., Segall, J., & Condeelis, J. (2011). High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harbor Protocols, 6(10), pp. 1167-1184. doi: 10.1101/pdb.top065904

  • Wyckoff, J., Gligorijevic, B., Entenberg, D., Segall, J., & Condeelis, J. (2011). The in vivo invasion assay: Preparation and handling of collection needles. Cold Spring Harbor Protocols, 6(10), pp. 1232-1234. doi: 10.1101/pdb.prot065912

  • Roussos, E.T., Balsamo, M., Alford, S.K., Wyckoff, J.B., Gligorijevic, B., Wang, Y., Pozzuto, M., Stobezki, R., Goswami, S., Segall, J.E., Lauffenburger, D.A., Bresnick, A.R., Gertler, F.B., & Condeelis, J.S. (2011). Mena invasive (Mena INV) promotes multicellular streaming motility and transendothelial migration in a mouse model of breast cancer. Journal of Cell Science, 124(13), pp. 2120-2131. doi: 10.1242/jcs.086231

  • Padgen, M.R., Raja, W.K., Gligorijevic, B., Williams, J.K., Condeelis, J.S., & Castracane, J. (2011). Complementary approaches to investigating cancer cell dynamics in the tumor microenvironment. Proceedings of SPIE - the International Society for Optical Engineering, 7929. doi: 10.1117/12.873421

  • Dovas, A., Gligorijevic, B., Chen, X., Entenberg, D., Condeelis, J., & Cox, D. (2011). Visualization of actin polymerization in invasive structures of macrophages and carcinoma cells using photoconvertible ß-actin - Dendra2 fusion proteins. PLoS ONE, 6(2). doi: 10.1371/journal.pone.0016485

  • Raja, W.K., Gligorijevic, B., Padgen, M.R., Eggers, D., Condeelis, J., & Castracane, J. (2010). Device for in-vivo study of the tumor microenvironment. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7593. doi: 10.1117/12.841338

  • Raja, W.K., Gligorijevic, B., Wyckoff, J., Condeelis, J.S., & Castracane, J. (2010). A new chemotaxis device for cell migration studies. Integrative Biology, 2(11-12), pp. 696-706. doi: 10.1039/c0ib00044b

  • Gligorijevic, B., Entenberg, D., Kedrin, D., Segall, J., Rheenen, J.v., & Condeelis, J. (2010). Intravital Imaging and Photoswitching in Tumor Invasion and Intravasation Microenvironments. Microscopy Today, 18(1), pp. 34-37. Cambridge University Press (CUP). doi: 10.1017/s1551929510991220

  • Gligorijevic, B., Kedrin, D., Segall, J.E., Condeelis, J., & Rheenen, J.v. (2009). Dendra2 photoswitching through the Mammary Imaging Window. Journal of Visualized Experiments : JoVE.(28).

  • Gligorijevic, B., Entenberg, D., Kedrin, D., Segall, J., Rheenen, J.V., & Condeelis, J. (2009). Intravital imaging and photomanipulation of tumor invasion and intravasation microenvironments. Microscopy and Microanalysis, 15(SUPPL. 2), pp. 86-87. doi: 10.1017/S143192760909312X

  • Raja, W.K., Gligorijevic, B., Condeelis, J.S., & Castracane, J. (2009). A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7207. doi: 10.1117/12.809366

  • Gligorijevic, B. & Condeelis, J. (2009). Stretching the timescale of intravital imaging in tumors. Cell Adhesion and Migration, 3(4). doi: 10.4161/cam.3.4.9581

  • Gligorijevic, B., Kedrin, D., Segall, J.E., Condeelis, J., & Rheenen, J.v. (2009). Dendra2 photoswitching through the mammary imaging window. Journal of Visualized Experiments.(28). doi: 10.3791/1278

  • Gligorijevic, B. & Condeelis, J. (2009). Stretching the timescale of intravital imaging in tumors. Cell Adhesion & Migration, 3(4), pp. 313-315. doi: 10.4161/cam.3.4.9581

  • Raja, W.K., Cady, N.C., Castracane, J., Gligorijevic, B., Rheenen, J.V., & Condeelis, J.S. (2008). The NANIVID: A new device for cancer cell migration studies. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 6859. doi: 10.1117/12.760730

  • Kedrin, D., Gligorijevic, B., Wyckoff, J., Verkhusha, V.V., Condeelis, J., Segall, J.E., & Rheenen, J.v. (2008). Intravital imaging of metastatic behavior through a mammary imaging window. Nature Methods, 5(12), pp. 1019-1021. doi: 10.1038/nmeth.1269

  • Gligorijevic, B., Purdy, K., Elliott, D.A., Cooper, R.A., & Roepe, P.D. (2008). Stage independent chloroquine resistance and chloroquine toxicity revealed via spinning disk confocal microscopy. Molecular and Biochemical Parasitology, 159(1), pp. 7-23. doi: 10.1016/j.molbiopara.2007.12.014

  • Gligorijevic, B., Purdy, K., & Roepe, P.D. (2007). Stage-specificity of antimalarial drug pharmacology revealed via quantitative 4D spinning disc confocal microscopy. BIOPHYSICAL JOURNAL, pp. 157A-157A. Retrieved from http://gateway.webofknowledge.com/

  • Gligorijevic, B., McAllister, R., Urbach, J.S., & Roepe, P.D. (2006). Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 1. Quantification of hemozoin development for drug sensitive versus resistant malaria. Biochemistry, 45(41), pp. 12400-12410. doi: 10.1021/bi061033f

  • Gligorijevic, B., Bennett, T., McAllister, R., Urbach, J.S., & Roepe, P.D. (2006). Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 2. Altered vacuolar volume regulation in drug resistant malaria. Biochemistry, 45(41), pp. 12411-12423. doi: 10.1021/bi0610348

  • Bennett, T.N., Paguio, M., Gligorijevic, B., Seudieu, C., Kosar, A.D., Davidson, E., & Roepe, P.D. (2004). Novel, Rapid, and Inexpensive Cell-Based Quantification of Antimalarial Drug Efficacy. Antimicrobial Agents and Chemotherapy, 48(5), pp. 1807-1810. doi: 10.1128/AAC.48.5.1807-1810.2004

  • Perrin, L., Bayarmagnai, B., Tüzel, E., & Gligorijevic, B. Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Cold Spring Harbor Laboratory. doi: 10.1101/2021.02.13.431047

  • Perrin, L., Bayarmagnai, B., Tuzel, E., & Gligorijevic, B. Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Research Square Platform LLC. doi: 10.21203/rs.3.rs-632734/v1