Bojana Gligorijevic

Bojana Gligorijevic

  • College of Engineering

    Bioengineering

    Associate Professor

  • Fox Chase Cancer Center

    Honorary Associate Professor

  • Lewis Katz School of Medicine

    Fels Cancer Institute for Personalized Medicine

    Associate Professor

Office Location

Engineering 810

Phone

215-204-3883

Biography

Bojana Gligorijevic, PhD, graduated Chemistry at University of Belgrade with an honors thesis in analytical chemistry, isolating active compounds from endemic plants. At Georgetown University, Dr. Gligorijevic’s PhD chemistry thesis took a turn when she first saw a fluorescent microscope. She ended up developing new technologies for automated 4D imaging and analysis of malaria division and metabolic processes. During her postdoctoral training in cancer biology at Albert Einstein College of Medicine, she developed new tools for intravital imaging in mouse models and contributed to the current view of tumor cell intravasation mechanism in tumor microenvironment context.

Labs: Cancer Microscopy & Mechanobiology Lab

Research Interests

  • Intravital imaging
  • Multiphoton Microscopy
  • Machine Learning Image Classification
  • Fluorescent Proteins
  • Cancer Complexity
  • Cell Decision-making
  • Extracellular Matrix Remodeling
  • Tumor Microenvironments
  • Mouse Models of Metastasis
  • Invadopodia
  • Motility & Invasion.

Courses Taught

Number

Name

Level

BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology

Undergraduate

BIOE 4555

Capstone Elective - Biophotonics: Seeing is Believing

Undergraduate

BIOE 5555

Biophotonics: Seeing is Believing

Graduate

Selected Publications

Featured

  • Pourfarhangi, K.E., Hoz, E.C.D.e.L.a., Cohen, A.R., & Gligorijevic, B. (2018). Contact guidance is cell cycle-dependent. APL Bioeng, 2(3). United States. doi: 10.1063/1.5026419

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2018). ECM Cross-Linking Regulates Invadopodia Dynamics. Biophys J, 114(6), pp. 1455-1466. United States. doi: 10.1016/j.bpj.2018.01.027

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., & Gligorijevic, B. (2018). Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context. Methods Mol Biol, 1749, pp. 175-193. United States. doi: 10.1007/978-1-4939-7701-7_14

  • Tönisen, F., Perrin, L., Bayarmagnai, B., Dries, K.v.d., Cambi, A., & Gligorijevic, B. (2017). EP4 receptor promotes invadopodia and invasion in human breast cancer. Eur J Cell Biol, 96(2), pp. 218-226. Germany. doi: 10.1016/j.ejcb.2016.12.005

  • Bergman, A. & Gligorijevic, B. (2015). Niche construction game cancer cells play. Eur Phys J Plus, 130(10). Germany. doi: 10.1140/epjp/i2015-15203-5

Recent

  • Perrin, L. & Gligorijevic, B. (2022). Proteolytic and mechanical remodeling of the extracellular matrix by invadopodia in cancer. Phys Biol, 20(1). England. doi: 10.1088/1478-3975/aca0d8

  • Perrin, L., Belova, E., Bayarmagnai, B., Tüzel, E., & Gligorijevic, B. (2022). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Commun Biol, 5(1), p. 758. England. doi: 10.1038/s42003-022-03642-z

  • Perrin, L., Bayarmagnai, B., Tuzel, E., & Gligorijevic, B. (2021). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. doi: 10.21203/rs.3.rs-632734/v1

  • Perrin, L., Belova, E., Bayarmagnai, B., Tüzel, E., & Gligorijevic, B. (2021). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. doi: 10.1101/2021.02.13.431047

  • Perrin, L., Tucker, T., & Gligorijevic, B. (2021). Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model. J Vis Exp.(167). United States. doi: 10.3791/61902

  • Firestein, R., Marcinkiewicz, C., Nie, L., Chua, H.K., Quesada, I.V., Torelli, M., Sternberg, M., Gligorijevic, B., Shenderova, O., Schirhagl, R., & Feuerstein, G.Z. (2021). Pharmacodynamic Studies of Fluorescent Diamond Carriers of Doxorubicin in Liver Cancer Cells and Colorectal Cancer Organoids. Nanotechnol Sci Appl, 14, pp. 139-159. New Zealand. doi: 10.2147/NSA.S321725

  • Gligorijevic, B. (2020). Real-time, high-resolution microscopy of invasive cancer cells in the tumor microenvironment context. J Biomol Tech, 31(Suppl), p. S40. United States. Retrieved from https://www.ncbi.nlm.nih.gov/

  • Perrin, L., Bayarmagnai, B., & Gligorijevic, B. (2020). Frontiers in Intravital Multiphoton Microscopy of Cancer. Cancer Rep (Hoboken), 3(1), p. e1192. United States. doi: 10.1002/cnr2.1192

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., Graña, X., Tüzel, E., & Gligorijevic, B. (2019). Invadopodia-mediated ECM degradation is enhanced in the G1 phase of the cell cycle. J Cell Sci, 132(20). England. doi: 10.1242/jcs.227116

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2019). Invadopodia Dynamics is Regulated by ECM Cross-Linking. BIOPHYSICAL JOURNAL, 116(3), pp. 414A-414A. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E. & Gligorijevic, B. (2019). Machine Learning Classification of Cancer Cells Migration in 3D Multi-cue Microenvironments 1. 2018 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2018 - Proceedings. doi: 10.1109/SPMB.2018.8615608

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., & Gligorijevic, B. (2018). lnvadopodia are enriched in the G1 phase of the cell cycle. MOLECULAR BIOLOGY of the CELL, 29(26), pp. 227-227. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E., Bayarmagnai, B., Perrin, L., & Gligorijevic, B. (2018). Integrin beta 1 is the master regulator of the Invadopodia state in breast carcinoma cells. MOLECULAR BIOLOGY of the CELL, 29(26). Retrieved from http://gateway.webofknowledge.com/

  • Perrin, L. & Gligorijevic, B. (2018). EMT is decoupled from invadopodia and metastasis. MOLECULAR BIOLOGY of the CELL, 29(26), pp. 225-226. Retrieved from http://gateway.webofknowledge.com/

  • Pourfarhangi, K.E., Hoz, E.C.D.e.L.a., Cohen, A.R., & Gligorijevic, B. (2018). Contact guidance is cell cycle-dependent. MOLECULAR BIOLOGY of the CELL, 29(26). Retrieved from http://gateway.webofknowledge.com/

  • Soboloff, J., Gligorijevic, B., & Zaidi, M.R. (2018). STIM1 (c)AMPs up melanogenesis. EMBO J, 37(5). England. doi: 10.15252/embj.201899047

  • Bergman, A. & Gligorijevic, B. (2018). Real-time imaging of invadopodia in tumor microenvironment context. Optics InfoBase Conference Papers, Part F89-MICROSCOPY 2018. doi: 10.1364/MICROSCOPY.2018.MF3A.3

  • Pourfarhangi, K.E., Bayarmagnai, B., Hoz, E.C.d.e.l.a., Cohen, A., & Gligorijevic, B. (2018). A methodology to investigate the relationship between cancer cells cell-cycle phase and their migratory behaviors. Optics InfoBase Conference Papers, Part F91-TRANSLATIONAL 2018. doi: 10.1364/TRANSLATIONAL.2018.JTu3A.13

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2017). Integrating live-cell fluorescent microscopy and signal processing to discover the relationship of invadopodia digging cycles with extracellular matrix crosslinking ratio. 2016 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2016 - Proceedings. doi: 10.1109/SPMB.2016.7846865

  • Perrin, L. & Gligorijevic, B. (2017). Role of Laminin Matrikines in Phenotypic Switching of Motile Cancer Cells. MOLECULAR BIOLOGY of the CELL, 28. Retrieved from http://gateway.webofknowledge.com/

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., & Gligorijevic, B. (2017). Invadopodia are limited to the G1 phase of cell cycle. MOLECULAR BIOLOGY of the CELL, 28. Retrieved from http://gateway.webofknowledge.com/

  • Bergman, A., Condeelis, J., & Gligorijevic, B. (2016). Invadopodium formation is enriched in perivascular niches and leads to cancer cell intravasation. CANCER RESEARCH, 76. doi: 10.1158/1538-7445.FBCR15-B05

  • Gligorijevic, B., Condeelis, J.S., & Bergman, A. (2014). Tumor cell motility in microenvironment context: From intravital microscopy to systems view. MOLECULAR BIOLOGY of the CELL, 25. Retrieved from http://gateway.webofknowledge.com/

  • Gligorijevic, B., Bergman, A., & Condeelis, J. (2014). Multiparametric classification links tumor microenvironments with tumor cell phenotype. PLoS Biol, 12(11), p. e1001995. United States. doi: 10.1371/journal.pbio.1001995

  • Génot, E. & Gligorijevic, B. (2014). Invadosomes in their natural habitat. Eur J Cell Biol, 93(10-12), pp. 367-379. Germany. doi: 10.1016/j.ejcb.2014.10.002

  • Bergman, A., Condeelis, J.S., & Gligorijevic, B. (2014). Invadopodia in context. Cell Adh Migr, 8(3), pp. 273-279. United States. doi: 10.4161/cam.28349

  • Gligorijevic, B., Bergman, A., Gil-Henn, H., & Condeelis, J. (2012). Microenvironments of tumor cell intravasation. CANCER RESEARCH, 72. doi: 10.1158/1538-7445.AM2012-2474

  • Gligorijevic, B., Wyckoff, J., Yamaguchi, H., Wang, Y., Roussos, E.T., & Condeelis, J. (2012). N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors. J Cell Sci, 125(Pt 3), pp. 724-734. England. doi: 10.1242/jcs.092726

  • Hulit, J., Kedrin, D., Gligorijevic, B., Entenberg, D., Wyckoff, J., Condeelis, J., & Segall, J.E. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. Methods Mol Biol, 872, pp. 15-30. United States. doi: 10.1007/978-1-61779-797-2_2

  • Wyckoff, J., Gligorijevic, B., Entenberg, D., Segall, J., & Condeelis, J. (2011). High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harb Protoc, 2011(10), pp. 1167-1184. United States. doi: 10.1101/pdb.top065904

  • Wyckoff, J., Gligorijevic, B., Entenberg, D., Segall, J., & Condeelis, J. (2011). The in vivo invasion assay: preparation and handling of collection needles. Cold Spring Harb Protoc, 2011(10), pp. 1232-1234. United States. doi: 10.1101/pdb.prot065912

  • Entenberg, D., Wyckoff, J., Gligorijevic, B., Roussos, E.T., Verkhusha, V.V., Pollard, J.W., & Condeelis, J. (2011). Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging. Nat Protoc, 6(10), pp. 1500-1520. England. doi: 10.1038/nprot.2011.376

  • Roussos, E.T., Balsamo, M., Alford, S.K., Wyckoff, J.B., Gligorijevic, B., Wang, Y., Pozzuto, M., Stobezki, R., Goswami, S., Segall, J.E., Lauffenburger, D.A., Bresnick, A.R., Gertler, F.B., & Condeelis, J.S. (2011). Mena invasive (MenaINV) promotes multicellular streaming motility and transendothelial migration in a mouse model of breast cancer. J Cell Sci, 124(Pt 13), pp. 2120-2131. England. doi: 10.1242/jcs.086231

  • Padgen, M.R., Raja, W.K., Gligorijevic, B., Williams, J.K., Condeelis, J.S., & Castracane, J. (2011). Complementary approaches to investigating cancer cell dynamics in the tumor microenvironment. Proceedings of SPIE - the International Society for Optical Engineering, 7929. doi: 10.1117/12.873421

  • Dovas, A., Gligorijevic, B., Chen, X., Entenberg, D., Condeelis, J., & Cox, D. (2011). Visualization of actin polymerization in invasive structures of macrophages and carcinoma cells using photoconvertible β-actin-Dendra2 fusion proteins. PLoS One, 6(2), p. e16485. United States. doi: 10.1371/journal.pone.0016485

  • Raja, W.K., Gligorijevic, B., Padgen, M.R., Eggers, D., Condeelis, J., & Castracane, J. (2010). Device for in-vivo study of the tumor microenvironment. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7593. doi: 10.1117/12.841338

  • Raja, W.K., Gligorijevic, B., Wyckoff, J., Condeelis, J.S., & Castracane, J. (2010). A new chemotaxis device for cell migration studies. Integr Biol (Camb), 2(11-12), pp. 696-706. England. doi: 10.1039/c0ib00044b

  • Gligorijevic, B., Entenberg, D., Kedrin, D., Segall, J., Rheenen, J.v., & Condeelis, J. (2010). Intravital Imaging and Photoswitching in Tumor Invasion and Intravasation Microenvironments. Micros Today, 18(1), pp. 34-37. United States. doi: 10.1017/S1551929510991220

  • Gligorijevic, B., Kedrin, D., Segall, J.E., Condeelis, J., & Rheenen, J.v. (2009). Dendra2 photoswitching through the Mammary Imaging Window. Journal of Visualized Experiments : JoVE.(28).

  • Gligorijevic, B., Entenberg, D., Kedrin, D., Segall, J., Rheenen, J.V., & Condeelis, J. (2009). Intravital imaging and photomanipulation of tumor invasion and intravasation microenvironments. Microscopy and Microanalysis, 15(SUPPL. 2), pp. 86-87. doi: 10.1017/S143192760909312X

  • Gligorijevic, B., Kedrin, D., Segall, J.E., Condeelis, J., & Rheenen, J.v. (2009). Dendra2 photoswitching through the Mammary Imaging Window. J Vis Exp.(28). United States. doi: 10.3791/1278

  • Raja, W.K., Gligorijevic, B., Condeelis, J.S., & Castracane, J. (2009). A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7207. doi: 10.1117/12.809366

  • Gligorijevic, B. & Condeelis, J. (2009). Stretching the timescale of intravital imaging in tumors. Cell Adhesion & Migration, 3(4), pp. 313-315. doi: 10.4161/cam.3.4.9581

  • Gligorijevic, B. & Condeelis, J. (2009). Stretching the timescale of intravital imaging in tumors. Cell Adh Migr, 3(4), pp. 313-315. United States. doi: 10.4161/cam.3.4.9581

  • Kedrin, D., Gligorijevic, B., Wyckoff, J., Verkhusha, V.V., Condeelis, J., Segall, J.E., & Rheenen, J.v. (2008). Intravital imaging of metastatic behavior through a mammary imaging window. Nat Methods, 5(12), pp. 1019-1021. United States. doi: 10.1038/nmeth.1269

  • Raja, W.K., Cady, N.C., Castracane, J., Gligorijevic, B., Rheenen, J.V., & Condeelis, J.S. (2008). The NANIVID: A new device for cancer cell migration studies. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 6859. doi: 10.1117/12.760730

  • Gligorijevic, B., Purdy, K., Elliott, D.A., Cooper, R.A., & Roepe, P.D. (2008). Stage independent chloroquine resistance and chloroquine toxicity revealed via spinning disk confocal microscopy. Mol Biochem Parasitol, 159(1), pp. 7-23. Netherlands. doi: 10.1016/j.molbiopara.2007.12.014

  • Gligorijevic, B., Purdy, K., & Roepe, P.D. (2007). Stage-specificity of antimalarial drug pharmacology revealed via quantitative 4D spinning disc confocal microscopy. BIOPHYSICAL JOURNAL, pp. 157A-157A. Retrieved from http://gateway.webofknowledge.com/

  • Gligorijevic, B., McAllister, R., Urbach, J.S., & Roepe, P.D. (2006). Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 1. Quantification of hemozoin development for drug sensitive versus resistant malaria. Biochemistry, 45(41), pp. 12400-12410. United States. doi: 10.1021/bi061033f

  • Gligorijevic, B., Bennett, T., McAllister, R., Urbach, J.S., & Roepe, P.D. (2006). Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 2. Altered vacuolar volume regulation in drug resistant malaria. Biochemistry, 45(41), pp. 12411-12423. United States. doi: 10.1021/bi0610348

  • Bennett, T.N., Paguio, M., Gligorijevic, B., Seudieu, C., Kosar, A.D., Davidson, E., & Roepe, P.D. (2004). Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy. Antimicrob Agents Chemother, 48(5), pp. 1807-1810. United States. doi: 10.1128/AAC.48.5.1807-1810.2004