Nancy Pleshko

Woman in dark room staring head on.

Nancy Pleshko

  • College of Engineering

    • Bioengineering

      • Professor Emerita

      • Adjunct Research Professor

Dr. Nancy Pleshko is a Professor Emerita and Adjunct Research Professor in the Department of Bioengineering at Temple University, Philadelphia, PA. She received her BSc degree in Chemistry from McGill University, Montreal, Canada, and her PhD in Chemistry from Rutgers University, NJ, in the field of spectroscopic applications to biomineralization. She was the director of the Musculoskeletal Imaging and Spectroscopy Laboratory at the Hospital for Special Surgery, an affiliate of Weill Cornell Medical School in New York City, for 15 years prior to joining Temple University in 2009.

Dr. Pleshko’s research lab (The Tissue Imaging and Spectroscopy Lab) focuses on advanced optical and vibrational spectroscopic technologies to address challenges in biomedical research and clinical diagnostics. She investigates tissues at the molecular, cellular, and structural levels to better understand tissue development, extracellular matrix composition, disease processes, and responses to therapeutic interventions. Her work spans translational studies of connective tissue pathophysiology and more recently environmental contaminants of concern including microplastics. She is a member of the Editorial Board for the journal Applied Spectroscopy, and is a Fellow of the Orthopedic Research Society and of the American Institute for Medical and Biological Engineering (AIMBE). She has received continuous federal funding for over 25 years and has published over 170 peer-reviewed articles.

Research Interests:

  • Biomedical Spectroscopy and Molecular Imaging: Development and application of advanced optical and vibrational spectroscopic techniques for biological tissue characterization.
  • Musculoskeletal Tissue Biology and Pathology: Investigation of bone and cartilage composition, structure, development, disease, and therapeutic responses.
  • Translational Optical Technologies: Development of minimally invasive and noninvasive fiber-optic probes for real-time tissue assessment and clinical decision-making.
  • Emerging Applications of Biospectroscopy: Non-destructive characterization of tissue-engineered constructs and detection and analysis of microplastics in biological tissues.

Selected Publications:

Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections. HassanMazandarani A, Masterson JM, Querido W, Steplewski A, Zhang Y, Huynh C, Garcia MM, Fertala A, Pleshko N. Analyst. 2026 Mar 17;151(6):1774-1785. doi: 10.1039/d5an00241a.

Optimization of Data Collection for Visible-Near-Infrared Fiber Optic Spectroscopy of Osteochondral Tissues in Hydrated Environments. Spurri A, Arefin MS, Querido W, Kust SJ, Santos M, Schaer TP, Fentaw B, Proca D, Barnes L, Patil C, Pleshko N. Appl Spectrosc. 2026 May;80(5):433-445. doi: 10.1177/00037028251411328.

Infrared Spectroscopy for Determining Bone Graft Healing and Readiness in Tissue Models.Di Pede R, Spurri A, Kust SJ, Block J, Querido W, Santana R, Semeniuk D, Pleshko N.Int J Oral Maxillofac Implants. 2026 Jul 31;0(0):1-27. doi: 10.11607/jomi.11616.

The Multifactorial Relationship Between Bone Tissue Water and Stiffness at the Proximal Femur. Querido W, Shanas N, Radway AP, Jones BC, Ispiryan M, Zhao H, Hast MW, Rajapakse CS, Pleshko N. Calcif Tissue Int. 2025 Jan 23;116(1):33. doi: 10.1007/s00223-024-01327-9.

Application of Optical Photothermal Infrared (O-PTIR) Spectroscopy for Assessment of Bone Composition at the Submicron Scale. Reiner E, Weston F, Pleshko N, Querido W. Appl Spectrosc. 2023 Nov;77(11):1311-1324. doi: 10.1177/00037028231201427