Dr. William Querido is a Research Assistant Professor in Bioengineering at Temple University. His research background is multidisciplinary, including two PhD degrees—in Biomedical Sciences (Biophysics) and Cell Biology, and significant postdoctoral training in fields of biomineralization, skeletal tissue engineering, and biospectroscopy. His research focuses on tissue mineralization in health and disease, with the ultimate goal of translating this knowledge into clinically-relevant outcomes to aid in the diagnosis and treatment of skeletal disorders. He specializes in applications of infrared spectroscopy and imaging modalities to explore the fascinating chemical and molecular mechanisms and complexities of bone development, aging, disease and regeneration. His main ongoing projects aim to reach a deeper understanding on how bone apatite composition and collagen integrity are associated with osteoporosis and bone strength. His research is currently funded by NIH/NIAMS (R21) and PA Department of Health (PA CURE).

Google Scholar: Google Scholar

Labs: Lab website

Research Interests

  • Chemistry and biology of mineralized tissues in health and disease
  • biomedical applications of infrared spectroscopy and spectral imaging
  • skeletal tissue engineering
  • cell-matrix-biomaterial interface

Courses Taught




BIOE 0844

The Bionic Human


BIOE 0944

Honors Bionic Human


Selected Publications


  • Ohnishi, T., Tran, V., Sao, K., Ramteke, P., Querido, W., Barve, R.A., Wetering, K.v.d.e., & Risbud, M.V. (2023). Loss of function mutation in Ank causes aberrant mineralization and acquisition of osteoblast-like-phenotype by the cells of the intervertebral disc. Cell Death Dis, 14(7), p. 447. England. doi: 10.1038/s41419-023-05893-y

  • Kim, M., Koyama, E., Saunders, C.M., Querido, W., Pleshko, N., & Pacifici, M. (2022). Synovial joint cavitation initiates with microcavities in interzone and is coupled to skeletal flexion and elongation in developing mouse embryo limbs. Biol Open, 11(6). England. doi: 10.1242/bio.059381

  • Querido, W., Zouaghi, S., Padalkar, M., Morman, J., Falcon, J., Kandel, S., & Pleshko, N. (2022). Nondestructive assessment of tissue engineered cartilage based on biochemical markers in cell culture media: application of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Analyst, 147(8), pp. 1730-1741. England. doi: 10.1039/d1an02351a

  • Shanas, N., Querido, W., Oswald, J., Jepsen, K., Carter, E., Raggio, C., & Pleshko, N. (2022). Infrared Spectroscopy-Determined Bone Compositional Changes Associated with Anti-Resorptive Treatment of the oim/oim Mouse Model of Osteogenesis Imperfecta. Appl Spectrosc, 76(4), pp. 416-427. United States. doi: 10.1177/00037028211055477

  • Kandel, S., Querido, W., Falcon, J.M., Zlotnick, H.M., Locke, R.C., Stoeckl, B., Patel, J.M., Patil, C.A., Mauck, R.L., & Pleshko, N. (2022). In Situ Assessment of Porcine Osteochondral Repair Tissue in the Visible-Near Infrared Spectral Region. Front Bioeng Biotechnol, 10, p. 885369. Switzerland. doi: 10.3389/fbioe.2022.885369

  • Falcon, J.M., Chirman, D., Veneziale, A., Morman, J., Bolten, K., Kandel, S., Querido, W., Freeman, T., & Pleshko, N. (2021). DMOG Negatively Impacts Tissue Engineered Cartilage Development. Cartilage, 13(2_suppl), pp. 722S-733S. United States. doi: 10.1177/1947603520967060

  • Ailavajhala, R., Querido, W., Rajapakse, C.S., & Pleshko, N. (2020). Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water. Analyst, 145(10), pp. 3713-3724. England. doi: 10.1039/c9an02491c

  • Shanas, N., Querido, W., Dumont, A., Yonko, E., Carter, E., Ok, J., Karchner, J.P., Barbe, M.F., Ali, S., Patil, C., Raggio, C., & Pleshko, N. (2020). Clinical application of near infrared fiber optic spectroscopy for noninvasive bone assessment. J Biophotonics, 13(4), p. e201960172. Germany. doi: 10.1002/jbio.201960172

  • Karchner, J.P., Querido, W., Kandel, S., & Pleshko, N. (2019). Spatial correlation of native and engineered cartilage components at micron resolution. Ann N Y Acad Sci, 1442(1), pp. 104-117. United States. doi: 10.1111/nyas.13934