Dr. Nancy Pleshko is a Professor in the Department of Bioengineering at Temple University, Philadelphia, PA. Dr. Pleshko is recognized for her research on assessment of tissues at the molecular, cellular, and structural level through application of state-of-the-art vibrational spectroscopy. She has substantial expertise in basic and translational research in connective tissue pathophysiology and orthopedics, including osteoporosis and other bone pathologies, and osteoarthritis and cartilage repair. She has over 90 publications, and has received over $5 million in federal funding.

Research Interests

  • Molecular Imaging of Tissues & Cells
  • Spectroscopic Analysis Techniques for Cartilage and Bone Pathologies
  • Tissue Engineering
  • Contribution of Molecular Alterations in Connective Tissue on Mechanical Behavior
  • MicroCT & Magnetic Resonance Imaging of Connective Tissues

Courses Taught




BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology


BIOE 3001

Research Design and Methods in Bioengineering


ENGR 9185

Exper Engineer Prof I


Selected Publications

  • Hong, A.L., Ispiryan, M., Padalkar, M.V., Jones, B.C., Batzdorf, A.S., Shetye, S.S., Pleshko, N., & Rajapakse, C.S. (2019). MRI-derived bone porosity index correlates to bone composition and mechanical stiffness. Bone Reports, 11. doi: 10.1016/j.bonr.2019.100213

  • Ailavajhala, R., Oswald, J., Rajapakse, C.S., & Pleshko, N. (2019). Environmentally-Controlled Near Infrared Spectroscopic Imaging of Bone Water. Scientific Reports, 9(1). doi: 10.1038/s41598-019-45897-3

  • Karchner, J.P., Querido, W., Kandel, S., & Pleshko, N. (2019). Spatial correlation of native and engineered cartilage components at micron resolution. Annals of the New York Academy of Sciences, 1442(1), pp. 104-117. doi: 10.1111/nyas.13934

  • Tint, D., Stabler, C.T., Hanifi, A., Yousefi, F., Linkov, G., Hy, K., Soliman, A., & Pleshko, N. (2019). Spectroscopic Analysis of Human Tracheal Tissue during Decellularization. Otolaryngology - Head and Neck Surgery (United States), 160(2), pp. 302-309. doi: 10.1177/0194599818806271

  • Jongwattanapisan, P., Terajima, M., Miguez, P.A., Querido, W., Nagaoka, H., Sumida, N., Gurysh, E.G., Ainslie, K.M., Pleshko, N., Perera, L., & Yamauchi, M. (2018). Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function. Scientific Reports, 8(1). doi: 10.1038/s41598-018-25279-x

  • Wischmann, J., Lenze, F., Thiel, A., Bookbinder, S., Querido, W., Schmidt, O., Burgkart, R., Eisenhart-Rothe, R.v., Richter, G., Pleshko, N., & Mayer-Kuckuk, P. (2018). Matrix mineralization controls gene expression in osteoblastic cells. Experimental Cell Research, 372(1), pp. 25-34. doi: 10.1016/j.yexcr.2018.09.005

  • Querido, W., Ailavajhala, R., Padalkar, M., & Pleshko, N. (2018). Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy. Applied Spectroscopy, 72(11), pp. 1581-1593. doi: 10.1177/0003702818789165

  • Yousefi, F., Kandel, S., & Pleshko, N. (2018). Infrared Spectroscopic Quantification of Methacrylation of Hyaluronic Acid: A Scaffold for Tissue Engineering Applications. Applied Spectroscopy, 72(10), pp. 1455-1466. doi: 10.1177/0003702818785353

  • Choi, H., Tessier, S., Silagi, E.S., Kyada, R., Yousefi, F., Pleshko, N., Shapiro, I.M., & Risbud, M.V. (2018). A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis. Matrix Biology, 70, pp. 102-122. doi: 10.1016/j.matbio.2018.03.019

  • Karchner, J.P., Yousefi, F., Bitman, S.R., Darvish, K., & Pleshko, N. (2018). Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties. Cartilage. doi: 10.1177/1947603518764269

  • Linkov, G., Hanifi, A., Yousefi, F., Tint, D., Bolla, S., Marchetti, N., Soliman, A., & Pleshko, N. (2018). Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy. Otolaryngology - Head and Neck Surgery (United States), 158(4), pp. 688-694. doi: 10.1177/0194599817752310

  • Yousefi, F., Kim, M., Nahri, S.Y., Mauck, R.L., & Pleshko, N. (2018). Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs. Tissue Engineering - Part A, 24(1-2), pp. 106-116. doi: 10.1089/ten.tea.2017.0035

  • Querido, W., Falcon, J.M., Kandel, S., & Pleshko, N. (2017). Vibrational spectroscopy and imaging: Applications for tissue engineering. Analyst, 142(21), pp. 4005-4017. doi: 10.1039/c7an01055a

  • Qu, D., Subramony, S.D., Boskey, A.L., Pleshko, N., Doty, S.B., & Lu, H.H. (2017). Compositional mapping of the mature anterior cruciate ligament-to-bone insertion. Journal of Orthopaedic Research, 35(11), pp. 2513-2523. doi: 10.1002/jor.23539

  • Rajapakse, C.S., Padalkar, M.V., Yang, H.J., Ispiryan, M., & Pleshko, N. (2017). Non-destructive NIR spectral imaging assessment of bone water: Comparison to MRI measurements. Bone, 103, pp. 116-124. doi: 10.1016/j.bone.2017.06.015

  • Doty, S.B., Bostrom, M.P., Bockman, R.S., Meulen, M.C.H.v.d., & Pleshko, N. (2017). Adele Ludin Boskey, PhD IN MEMORIAM. JOURNAL of BONE and MINERAL RESEARCH, 32(8), pp. 1597-1598. doi: 10.1002/jbmr.3187

  • Hanifi, A., Palukuru, U., McGoverin, C., Shockley, M., Frank, E., Grodzinsky, A., Spencer, R.G., & Pleshko, N. (2017). Near infrared spectroscopic assessment of developing engineered tissues: correlations with compositional and mechanical properties. Analyst, 142(8), pp. 1320-1332. doi: 10.1039/c6an02167k

  • Cheheltani, R., Pichamuthu, J.E., Rao, J., Weinbaum, J.S., Kiani, M.F., Vorp, D.A., & Pleshko, N. (2017). Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients. Cardiovascular Engineering and Technology, 8(1), pp. 70-80. doi: 10.1007/s13239-016-0289-3

  • Spencer, R.G., Cortese, B.D., Lukas, V.A., & Pleshko, N. (2017). Point Estimates of Test Sensitivity and Specificity from Sample Means and Variances. American Statistician, 71(1), pp. 81-87. doi: 10.1080/00031305.2016.1239589

  • Wolfson, M.R., Pleshko, N., Darvish, K.K., Baker, S.T., Wu, J., Kyada, R., Nelson, C., Fukuda, S., Enkhbaatar, P., Florova, G., Komissarov, A.A., Idell, S., & Shaffer, T.H. (2017). Differential Impact Of Fibrinolysins On Airway Cast Composition And Mechanical Properties Assessed By A Novel Infrared Imaging And Microindentation Approach. AMERICAN JOURNAL of RESPIRATORY and CRITICAL CARE MEDICINE, 195. Retrieved from

  • Gangolli, R.A., Devlin, S.M., Ailavajhala, R., Hanifi, A., Pleshko, N., Lelkes, P.I., & Yang, M. (2016). Biomimetic Scaffold to Regenerate the Pulp Dentin Complex. TISSUE ENGINEERING PART A, 22, pp. S72-S72. Retrieved from

  • Raggio, C.L., Pleshko, N., & Boskey, A.L. (2016). The Effect of Stontium Ranelate on Fracture Reduction in Osteogenesis Imperfecta is Comparable to Recent Bisphosphonate Data. Journal of Bone and Mineral Research, 31(11), p. 2065. doi: 10.1002/jbmr.2976

  • Eisenhauer, P., Chernets, N., Song, Y., Dobrynin, D., Pleshko, N., Steinbeck, M.J., & Freeman, T.A. (2016). Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation. Journal of Tissue Engineering and Regenerative Medicine, 10(9), pp. 772-782. doi: 10.1002/term.2224

  • Palukuru, U.P., Hanifi, A., McGoverin, C.M., Devlin, S., Lelkes, P.I., & Pleshko, N. (2016). Near infrared spectroscopic imaging assessment of cartilage composition: Validation with mid infrared imaging spectroscopy. Analytica Chimica Acta, 926, pp. 79-87. doi: 10.1016/j.aca.2016.04.031

  • Ashinsky, B.G., Fishbein, K.W., Carter, E.M., Lin, P.C., Pleshko, N., Raggio, C.L., & Spencer, R.G. (2016). Multiparametric classification of skin from osteogenesis imperfecta patients and controls by quantitative magnetic resonance microimaging. PLoS ONE, 11(7). doi: 10.1371/journal.pone.0157891

  • McGoverin, C.M., Hanifi, A., Palukuru, U.P., Yousefi, F., Glenn, P., Shockley, M., Spencer, R.G., & Pleshko, N. (2016). Nondestructive Assessment of Engineered Cartilage Composition by Near Infrared Spectroscopy. Annals of Biomedical Engineering, 44(3), pp. 680-692. doi: 10.1007/s10439-015-1536-8

  • Bozkurt, O., Bilgin, M.D., Evis, Z., Pleshko, N., & Severcan, F. (2016). Early Alterations in Bone Characteristics of Type i Diabetic Rat Femur: A Fourier Transform Infrared (FT-IR) Imaging Study. Applied Spectroscopy, 70(12), pp. 2005-2015. doi: 10.1177/0003702816671059

  • Cheng, H.A., Drinnan, C.T., Pleshko, N., & Fisher, O.Z. (2015). Pseudotannins self-assembled into antioxidant complexes. Soft Matter, 11(39), pp. 7783-7791. doi: 10.1039/c5sm01224d

  • Boskey, A.L., Marino, J., Spevak, L., Pleshko, N., Doty, S., Carter, E.M., & Raggio, C.L. (2015). Are Changes in Composition in Response to Treatment of a Mouse Model of Osteogenesis Imperfecta Sex-dependent? Clinical Orthopaedics and Related Research, 473(8), pp. 2587-2598. doi: 10.1007/s11999-015-4268-z

  • Padalkar, M.V. & Pleshko, N. (2015). Wavelength-dependent penetration depth of near infrared radiation into cartilage. Analyst, 140(7), pp. 2093-2100. doi: 10.1039/c4an01987c

  • Gollwitzer, H., Yang, X., Spevak, L., Lukashova, L., Nocon, A., Fields, K., Pleshko, N., Courtland, H.W., Bostrom, M.P., & Boskey, A.L. (2015). Fourier transform infrared spectroscopic imaging of fracture healing in the normal mouse. Journal of Spectroscopy, 2015. doi: 10.1155/2015/659473

  • Hemmasizadeh, A., Tsamis, A., Cheheltani, R., Assari, S., D'Amore, A., Autieri, M., Kiani, M.F., Pleshko, N., Wagner, W.R., Watkins, S.C., Vorp, D., & Darvish, K. (2015). Correlations between transmural mechanical and morphological properties in porcine thoracic descending aorta. Journal of the Mechanical Behavior of Biomedical Materials, 47, pp. 12-20. doi: 10.1016/j.jmbbm.2015.03.004

  • O’Brien, M.P., Penmatsa, M., Palukuru, U., West, P., Yang, X., Bostrom, M., Freeman, T., & Pleshko, N. (2015). Monitoring the Progression of Spontaneous Articular Cartilage Healing with Infrared Spectroscopy. Cartilage, 6(3), pp. 174-184. doi: 10.1177/1947603515572874

  • Palukuru, U.P., McGoverin, C.M., & Pleshko, N. (2014). Assessment of hyaline cartilage matrix composition using near infrared spectroscopy. Matrix Biology, 38, pp. 3-11. doi: 10.1016/j.matbio.2014.07.007

  • Terajima, M., Perdivara, I., Sricholpech, M., Deguchi, Y., Pleshko, N., Tomer, K.B., & Yamauchi, M. (2014). Glycosylation and cross-linking in bone type I collagen. Journal of Biological Chemistry, 289(33), pp. 22636-22647. doi: 10.1074/jbc.M113.528513

  • Cheheltani, R., McGoverin, C.M., Rao, J., Vorp, D.A., Kiani, M.F., & Pleshko, N. (2014). Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta. Analyst, 139(12), pp. 3039-3047. doi: 10.1039/c3an02371k

  • Johnson, C.M., Pleshko, N., Achary, M., & Suri, R. (2014). Rapid and sensitive screening of 17ß-estradiol estrogenicity using fourier transform infrared imaging spectroscopy (FT-IRIS). Environmental Science and Technology, 48(8), pp. 4581-4587. doi: 10.1021/es5000676

  • Padalkar, M., McGoverin, C., Onigbanjo, Q., Spencer, R., Barbash, S., Kropf, E., & Pleshko, N. (2014). Infrared fiber optic probes for evaluation of musculoskeletal tissue pathology. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 8926. doi: 10.1117/12.2047473

  • Barbe, M.F., Adiga, R., Gordiienko, O., Pleshko, N., Selzer, M.E., & Krynska, B. (2014). Micro-computed tomography assessment of vertebral column defects in retinoic acid-Induced rat model of myelomeningocele. Birth Defects Research Part a - Clinical and Molecular Teratology, 100(6), pp. 453-462. doi: 10.1002/bdra.23254

  • Sahu, A., Saleheen, F., Oleksyuk, V., McGoverin, C., Pleshko, N., Torbati, A., Picone, J., Sorenmo, K., & Won, C.H. (2014). Characterization of mammary tumors using noninvasive tactile and hyperspectral sensors. IEEE Sensors Journal, 14(10), pp. 3337-3344. doi: 10.1109/JSEN.2014.2323215

  • Khanarian, N.T., Boushell, M.K., Spalazzi, J.P., Pleshko, N., Boskey, A.L., & Lu, H.H. (2014). FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age. Journal of Bone and Mineral Research, 29(12), pp. 2643-2652. doi: 10.1002/jbmr.2284

  • McGoverin, C.M., Lewis, K., Yang, X., Bostrom, M., & Pleshko, N. (2014). The contribution of bone and cartilage to the near-infrared spectrum of osteochondral tissue. Applied Spectroscopy, 68(10), pp. 1168-1175. doi: 10.1366/13-07327

  • Caccese, N., Palukuru, U., Thakkar, P., McGoverin, C., Pleshko, N., Cheng, C., Solorio, L., Correa, D., Philips, L., McMillan, A., & Alsberg, E. (2014). FTIR imaging analysis of bioactive microsphere incorporated stem cell sheets for osteochondral defect repair. Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC, 2014-December. doi: 10.1109/NEBEC.2014.6972745